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YBLiHepato Demonstrate Hepatocyte-like Cells Morphology and Intracellular Lipid Accumulation
YBLiHepato cells exhibit a characteristic cobblestone morphology, with rounded nuclei that feature prominent nucleoli, as indicated by the arrow. Notably, the presence of bi-nucleation, as denoted by the ovals, further underscores the differentiation of these cells into hepatocyte-like cells. Furthermore, Oil Red O staining confirms the successful differentiation of iPSCs into hepatocyte-like cells, as it reveals the presence of lipid droplets, a key feature of mature hepatocytes. This observation highlights the promising potential of YBLiHepato cells in liver-related research and applications.
YBLiHepato: Cyp Activity (metabolite concentrations) by LC-MS/MS Method
Assay Conditions:
YBLiHepato cells exhibit CYP activity and metabolite formation compared to both HepG2 cells and Primary Human Hepatocytes (PHH). This underscores the utility of YBLiHepato cells in hepato research, offering a valuable resource for studying drug metabolism and toxicology in vitro.
YBLiHepato Demonstrates Cyp Activity and Metabolite Formation by LC-MS/MS Method
Data points are presented as the mean ± standard error of the mean (SEM) from four distinct experimental replicates; Unpaired two-tailed t-tests were conducted to assess statistical significance. Significant differences are denoted as follows: * p≤0.01 ** p≤0.001, *** p≤0.0005; **** p≤0.0001
YBLiHepato: Urea Formation by iPSC-derived Hepatocytes
YBLiHepato demonstrates active urea production, reflecting successful differentiation and functional maturation.
Urea secreted into the medium during a 24-hr period was measured by colorimetric in HepG2, YBLiHepato and primary human hepatocytes (PHH) on D2. Urea secretion in spent media was estimated by the Urea estimation kit (Cat. No. KA1652 ) Data represents the normalization of urea secretion to total cell number. Data is presented as the mean ±SEM (n=3). An unpaired two-tailed t-test was used to calculate the p-value; **p≤0.005
LDL Uptake and Cholesterol Metabolism in YBLiHepato
Data is presented as the mean ±SEM (n=5). An unpaired two-tailed t-test was used to calculate the p-value; p≤0.01
YBLiHepato: RT-qPCR Analysis of Genes
Data points are presented as the mean ± standard error of the mean (SEM) from three distinct experimental replicates; Unpaired two-tailed t-tests were conducted to assess statistical significance. Significant differences are denoted as follows: ** p≤0.001, ****p≤0.0001
YBLiHepato: RT-qPCR Analysis of Phase I and Phase II Enzyme
Data points are presented as the mean ± standard error of the mean (SEM) from three distinct experimental replicates; mean ± SEM (N=2, n=4); Unpaired two-tailed t-tests were conducted to assess statistical significance. Significant differences are denoted as follows: * p≤0.02, **** p≤0.0001
YBLiHepato - RT-qPCR Analysis of Bile Transporters
Graph showing the mean ± SEM (n=3); Unpaired two-tailed t-tests were conducted to assess statistical significance. Significant differences are denoted as follows: ** p≤0.001 *** p≤0.0005; **** p≤0.0001
YBLiHepato - RT-qPCR Analysis of Fatty Acid Metabolism Genes
Graph showing the mean ± SEM (n=3); Unpaired two-tailed t-tests were conducted to assess statistical significance. Significant differences are denoted as follows: ** p≤0.001 *** p≤0.0005; **** p≤0.0001
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