Human Peripheral Blood Mononuclear Cells (PBMCs)

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Peripheral Blood Mononuclear Cells are isolated from whole blood, leukapheresis, or whole blood samples using density gradient separation or red blood cell lysis and cryopreserved. All peripheral blood samples are obtained from donors diagnosed with cancer, autoimmune or lifestyle diseases with Institutional Ethics Committee (IEC)-approved consent forms/waivers and protocols. Sodium heparin/EDTA/Sodium Citrate is added during collection to the vacuutainer tube as an anticoagulant during peripheral blood collection. PBMCs form the starting source for isolation of several types of immune cells, HLA analysis as well as immune function

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Clone

Synonym

Host Species

Mouse

Target Species

Human

Isotype

Purity

>95%

Format

Liquid

Source

Applications

Affinity (M)

Application

Purity Analysis

Immunological Activity

Presentation

Donor Status

Healthy/Diseased

Characterization Criteria

Cell count, viability, age, sex, ethnicity, weight, height, smoking status, other information

Format

1 or 2 million PBMCs per vial cryopreserved in CryoStor® CS10

Anticoagulant

Sodium Heparin/Other as per specific requirement

Quantity

Storage

Liquid Nitrogen

Stability

Other Information

Viral Testing

Material Safety Note

Note- Donors are screened for HIV ½, HBV and HCV viral markers before blood collection

The PBMCs isolated from the Buffy coat are verified for their viability and purity (Refer Fig 1 below).

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Fig 1. Graphical representation of PBMCs’ viability in percentage that ranges from 70% to 100%.

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Fig 2. Representative flow cytometry plots showing the presence of CD45 immunophenotype of PBMCs derived from Human blood from three different patients and compared with a negative control cell line (HEK 293). The absolute PBMC cell population obtained from the buffy coat are represented in percentage (V1-R). The Isotype control depicts that the fluoro-chrome tagged antibody against CD45 is specific. NOTE: The above data is obtained after thawing cryopreserved PBMCs.

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